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During tooth development, enamel matrix proteins (EMP) secreted by the differentiating ameloblasts lead to the deposition of a mature and highly mineralized enamel matrix. The control of these processes is mainly exerted at the transcriptional level. We showed that tyrosine phosphorylation is involved in the maturation of the enamel matrix proteins Mmp20, Mmp28, and Mmp29. In this study we have investigated the signaling pathways that regulate the phosphorylation of tyrosine residues in both the microfibrillar and fine fibrillar enamel matrix proteins. We present a model of the signaling pathways that regulate the phosphorylation of tyrosine residues in the EMP of differentiating ameloblasts, which is compatible with previous data on the phosphorylation of tyrosine residues in actin and the activation of Erk1/2. This study further demonstrates that this signaling pathway might also be involved in the regulation of signaling to the nucleus, which was previously shown for the mineralization of enamel. We suggest that the coordinated signaling pathways activated by the secretion of EMP may eventually control ameloblast behavior at the transcriptional and posttranscriptional level and that they may directly involve the activation of Erk1/2.1. Field of the Invention
The present invention relates to a ground wire disconnection preventing apparatus, which is included in a ground wire disconnection detecting device for detecting that a ground wire of an electric car is disconnected from a ground wire rail of a power feeding side, and which stops the operation

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