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Hofmeister ions have been described as stabilizing agents for biological samples. Depending on the concentration and the type of ionic species, the Hofmeister series affects protein aggregation, cytoskeleton organization, mitochondrial function, and even platelet aggregation. However, these effects of Hofmeister ions have been mainly studied on proteins and cells, but they have also been reported for other biological samples such as, e.g., lipids, glucose, DNA. In this work, we studied the effect of Hofmeister cations on the size and structure of exosomes, which are extracellular vesicles released from cells. DLS and TEM analyses showed that the size of exosomes can be modulated by the Hofmeister ions type and their concentration. Specifically, our results demonstrate that the Hofmeister cations determined the size of the exosomes in the Hofmeister series of Cl⁻ > Na⁺ > K⁺ ≈ KCl and that the larger the concentration of Hofmeister cations, the larger the size of the exosomes. In addition, we demonstrated that Hofmeister ions affect the intracellular uptake efficiency of exosomes by different cell lines. Our results demonstrate the potential application of the Hofmeister ion series in the regulation of the size and structure of exosomes, which is an important parameter for their cellular uptake efficiency. are, though. It’s a cold stretch of nothing, actually. I thought the last few years would be different. But then, I thought by now we would be more self-reliant, and would know more
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