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The strategy outlined in this chapter is designed to be used for the identification of cDNA clones encoding Cytochrome P450 enzymes in a large library of cDNA clones. Initial screening for the activity of a P450 enzyme is achieved by screening for the formation of a metabolic product which may be detected by bioassay, or by immunoassay where a cDNA encoding the P450 enzyme is used as a probe to detect a specific mRNA that correlates with enzyme activity. In the case of cDNA library screening in yeast, the strategy relies on the ability to select mutant cell lines that have incorporated sufficient numbers of cDNA copies in the cell in order to enable the metabolic phenotype or immunoprecipitation of the relevant cDNA to be detected. The method described is generic and could be applied for the investigation of the functions of any P450 enzymes.Management of endometriosis-related dysmenorrhea using oral contraceptives.
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